Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: Top 20 differentially expressed lncRNAs in SNIP tissues detected by microarray assay.

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Microarray

Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: Top 20 differentially expressed mRNAs in SNIP tissues detected by microarray assay.

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Microarray

Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: qRT-PCR validation of eight differentially expressed lncRNAs and three mRNAs selected by the microarray assay. Comparison of lncRNA or mRNA expression levels obtained by microarray and qRT-PCR analysis. Upregulated and downregulated lncRNAs or mRNAs are indicated by bars above and below the horizontal axis, respectively. The data from qRT-PCR was shown as mean ± standard deviation (SD).

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Quantitative RT-PCR, Microarray, Expressing, Standard Deviation

Journal: Medicine

Article Title: Analysis of the long non-coding RNA LINC01614 in non-small cell lung cancer

doi: 10.1097/MD.0000000000016437

Figure Lengend Snippet: The procedure of total RNA transforming into single-strand DNA labeled with Cy3-dCTP or Cy5-dCTP. lncRNAs = long non-coding RNAs.

Article Snippet: 1ug total RNA was used to be transformed into single-strand DNA labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, Piscataway, NJ) through various steps (The procedure was depicted in Fig. ).The final products were hybridized to the CapitalBio Jingxin lncRNA&mRNA Human 4 × 180K Gene Expression Microarray V4.0 (CapitalBio Corp., Beijing, China), which contains 40,916 lncRNA detection probes and 34,235 mRNA detection probes.

Techniques: Labeling

Journal: Medicine

Article Title: Analysis of the long non-coding RNA LINC01614 in non-small cell lung cancer

doi: 10.1097/MD.0000000000016437

Figure Lengend Snippet: The procedure of total RNA transforming into single-strand DNA labeled with Cy3-dCTP or Cy5-dCTP. lncRNAs = long non-coding RNAs.

Article Snippet: 1ug total RNA was used to be transformed into single-strand DNA labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, Piscataway, NJ) through various steps (The procedure was depicted in Fig. ).The final products were hybridized to the CapitalBio Jingxin lncRNA&mRNA Human 4 × 180K Gene Expression Microarray V4.0 (CapitalBio Corp., Beijing, China), which contains 40,916 lncRNA detection probes and 34,235 mRNA detection probes.

Techniques: Labeling